Figure 2
From: Maintaining bovine satellite cells stemness through p38 pathway
(a) Representative immunofluorescent staining of DAPI, PAX7 and p-p38 in quiescent bovine satellite cells (freshly isolated fiber, top) and activated satellite cells in culture (bottom). Scale bars: 25 μm. (b) qRT-PCR: concentration-dependent inhibition of PAX7 expression in 2 days cultured satellite cells (passage 2) by p38i inhibitor SB203580. (n = 3). (c) Relative percentage of 1.5 hr window EdU incorporation at given concentrations of p38i for satellite cells at passage 2 after 2 days culturing. (n = 4). (d) Representative images of immunoblotting against p-HSP27, p-p38, p38, and GAPDH from cell lysates of cells cultured 4 days to 90% confluent in the presence or absence of 10 μM p38i. Full-length blots are presented in Supplementary Fig. S5. (e) Relative levels of p-HSP27 normalized to GAPDH are indicated from (d). (n = 3). (f) Relative levels of p-p38 normalized to p38 from (d). (n = 3). Data are represented as mean ± SEM. Significance was analyzed by Student’s t-test for 2 groups, One-way ANOVA with Bonferroni’s Multiple Comparison Test for more than 2 groups. Asterisks: *indicates P < 0.05, **indicates P < 0.01, ***indicates P < 0.001.
