Table 1 Numbers of cultures and cultured astrocytes in which calcium transients evoked by the application of WIN, anandamide and ATP were measured.

From: CB1 receptor activation induces intracellular Ca2+ mobilization and 2-arachidonoylglycerol release in rodent spinal cord astrocytes

animal type

drug

n (cultures)

n (cells responding to ATP)

n (cells responding to drug)

proportion of ATP sensitive cells responding to drug

amplitude of Ca2+ transient normalized to ATP response (%)

Wistar rat

WIN

15

233

10

4.3 ± 0.5

22.33 ± 5.52

AEA

10

122

6

4.9 ± 1.2

29.87 ± 14.48

C57BL/6 mouse

WIN

6

157

13

8.3 ± 0.7

21.61 ± 4.12

AEA

8

202

37

18.3 ± 1.2

27.37 ± 4.68

CB1-R KO mouse

WIN

9

400

10

2.5 ± 0.2

8.28 ± 1.01

AEA

12

505

27

5.3 ± 0.3

19.28 ± 2.14

  1. The proportion of astrocytes responding to the application of WIN, anandamide and ATP, as well as the amplitudes of the calcium transients recorded from the responding cells, normalized to ATP responses, are also presented. n: number, AEA: anandamide.
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