Figure 3

The anti-oxidative roles of Paeonin. (A) ARE-luciferase activity was measured by luciferase assay in H₂O₂-stimulated GES-1 cells with different Paeonin concentrations, including 20 μg/ml, 50 μg/ml, 100 μg/ml and 200 μg/ml. With the concentrations of Paeonin increased, the ARE-luciferase activity was also elevated. (B) HO-1 mRNA was determined by qRT-PCR in H₂O₂-incubated GES-1 cells with different Paeonin concentrations. HO-1 mRNA expressions and Paeonin concentrations had a positive correlation. (C) ARE-luciferase activity was detected by luciferase assay in H₂O₂-stimulated GES-1 cells with 200 μg/ml Paeonin for 1 h, 2 h, 4 h, 8 h, 12 h and 24 h. With the treatment time prolonged, the ARE-luciferase activity was also up-regulated. (D) HO-1 mRNA was tested by qRT-PCR in H₂O₂-incubated GES-1 cells with 200 μg/ml Paeonin for 1 h, 2 h, 4 h, 8 h, 12 h and 24 h. HO-1 mRNA expressions and the treatment time had a positive correlation; *P < 0.05 versus the H₂O₂ group.