Figure 3 | Scientific Reports

Figure 3

From: A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model

Figure 3

V407F stabilizes K405. (a) Overlaid crystal structures of WT (grey) and V407F (salmon) protein complexes show similar global conformation. (b) In MD simulations, the RMSF plot shows the difference in the structural flexibility of the residues between the WT and V407F mutant structures. (c) The difference RMSF plot (ΔRMSF = RMSF(V407F) − RMSF(WT)) shows the reduced structural flexibility of K405 in the IDF of the V407F mutant structure. (d) Neutralizing mutation K405N right-shifts the dose-dependent activation of the V407F mutant SK2-a channel by Ca2+. Statistical analysis was performed using two-tailed t-test. All data are presented in mean ± s.e.m.

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