Figure 5

Phenylalanine substitution of the cognate valine in SK1 and SK3 channels increases Ca²⁺ sensitivity. (a) Dose-dependent activation of the WT and V378F mutant SK1 current by Ca²⁺. (b) EC₅₀ values for the activation of the WT and mutant SK1 channels by Ca²⁺. (c) Dose-dependent activation of the WT and V506F mutant SK3 current by Ca²⁺. (d) EC₅₀ values for the activation of the WT and mutant SK3 channels by Ca²⁺. Statistical analysis was performed using two-tailed t-test. All data are presented in mean ± s.e.m.