Table 1 Genes identified as functional regulators of DiI-LDL uptake into cultured primary human monocyte-derived macrophages.

Gene	siRNA ID	Primary Screen			Replication Screen
Gene	siRNA ID	Mean Md(C)	Mean Md(P)	Mean Md(C + P)	Mean Md(C)	Mean Md(P)	Mean Md(C + P)
ALDH1A2	s16908	0.9	0.3	0.6	n.a.	n.a.	n.a.
	s225046	−0.2	−1.2	−0.5	−1.2	−1.8	−1.4
	s16907	0.8	−0.9	−0.3	−0.8	−0.9	−0.8
APOC1**	s194287	−1.4	−0.6	−1.2	−1.1	−1.5	−1.1
	s1481	0.1	0.1	0.1	n.a.	n.a.	n.a.
	s1482	−0.5	−0.8	−0.7	−0.9	−1.5	−1.0
CMTM6**	s29750	−1.0	0.0	−0.2	−1.0	−1.5	−1.2
	s226803	−0.7	−0.6	−0.6	−0.9	−1.6	−1.1
	s29749	0.3	0.3	0.3	n.a.	n.a.	n.a.
EPHX1	s4750	−0.1	1.5	0.2	−0.9	−1.6	−1.1
	s4749	0.5	0.9	0.7	−0.6	−0.4	−0.5
	s4751	−0.1	−0.2	−0.2	n.a.	n.a.	n.a.
FABP4**	s4965	0.0	0.5	0.2	n.a.	n.a.	n.a.
	s4966	−3.1	−2.6	−2.9	−1.2	−2.0	−1.3
	s4964	−0.6	−1.2	−1.0	−0.7	−1.3	−0.9
WBP5**	s27638	−0.8	0.2	−0.3	−1.1	−2.2	−1.4
	s27637	−0.3	0.3	−0.1	n.a.	n.a.	n.a.
	s27639	−1.1	−0.4	−0.7	−0.9	−1.9	−1.1
ZYX	s15351	−0.1	0.2	−0.1	n.a.	n.a.	n.a.
	s15352	0.8	−0.1	0.1	−0.4	−0.3	−0.3
	s224804	1.6	0.6	0.8	−0.5	−0.5	−0.5

The impact on DiI-LDL uptake upon candidate gene knockdown was analyzed with 3 independent siRNAs/gene. SiRNAs meeting significance criteria (z-score/Deviation >|1|) are displayed in bold as mean medians of deviations (Md). Genes with ≥2 significant siRNAs validated in replication screen are highlighted by**. Negative values indicate reduction in DiI-LDL uptake relative to control-siRNA transfected cells. C, control individuals; P, coronary-artery disease patients; column Md(C + P) indicates means across patients and control individuals in this study. n.a., not analyzed.

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