Figure 2 | Scientific Reports

Figure 2

From: O-GlcNAcylation of FoxO1 mediates nucleoside diphosphate kinase B deficiency induced endothelial damage

Figure 2

FoxO1 is upregulated in NDPK-B depleted ECs. (A) Representative immunoblots of FoxO1, Ang-2 and NDPK-B in NDPK-B depleted ECs. (B,C) Levels of FoxO1 and Ang-2 normalized to γ-tubulin (n = 7, **p < 0.01, ***p < 0.001). (D) Immunofluorescence staining of FoxO1 in NDPK-B depleted ECs (scale bar 10 µm). (E,F) Quantifications of FoxO1 in the cytoplasm and the nucleus (n = 3). Pixel densities were quantified using the Image J software. Quantitation of the FoxO1 density as obtained from analyzing 20 cells from randomly selected frames for each of three independent EC isolations. (E) Transcriptional analysis of FoxO1 by quantitative real-time PCR. The values in control are standardized to 1. n = 4.

Back to article page