Figure 2 | Scientific Reports

Figure 2

From: Wnt7a induces satellite cell expansion, myofiber hyperplasia and hypertrophy in rat craniofacial muscle

Figure 2

Wnt7a increases satellite cell proportion. (a) Schematic illustration of experimental procedures: Rat LVP, MAS, TA muscle were injected with rh-Wnt7a and were analyzed at day 21, 35, 56 after rh-Wnt7a administration. (b–d) Immunofluorescence staining of laminin (green), Pax7 (red) and DAPI (blue) in PBS-treated muscle groups. (b’–d’) Immunofluorescence staining Pax7 (red) in PBS-treated muscle groups. (e–g) Immunofluorescence staining of laminin (green), Pax7 (red) and DAPI (blue) in rh-Wnt7a-treated muscle groups. (e’–g’) Immunofluorescence staining Pax7 (red) in Wnt7a-treated muscle groups. (h) Quantification of Pax7+ve nuclei in PBS-treated and rh-Wnt7a-treated muscle groups at 21 days after injection. For each group, N = 6. **p < 0.01; ***p < 0.001.

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