Figure 1 | Scientific Reports

Figure 1

From: Transgenic Tmc2 expression preserves inner ear hair cells and vestibular function in mice lacking Tmc1

Figure 1

Generation of Rosa26tm(CAG-LSL-Tmc2-IRES-GFP) mouse model. (A) Mouse Tmc2 cDNA was inserted into the CAG-STOP-GFP-Rosa targeting vector, CTV, between a floxed Stop cassette and the internal ribosome entry site (IRES) followed by the enhanced Green Fluorescent Protein gene (eGFP). Transcription is under control of the CAG promoter. The targeting vector contained Rosa26 homology arms (1 kb 5′ and 3.8 kb 3′), so that the entire loxP-stop-loxP-Tmc2-IRES-GFP transcriptional cassette was inserted into the first intron of Rosa26 gene on chromosome 6. (B) When expressed with Gfi1Cre, excision of the stop codon lead to expression of Tm[Tmc2] and GFP in IHCs and OHCs. GFP expression was evident at P16. Hair cells were labeled with rabbit anti-MYOVIIa (red), GFP signal is amplified with a goat anti-GFP antibody (green) and phalloidin counterstain labeled actin filaments (blue). Scale bar 50 μm.

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