Figure 1
From: Fate of mesoangioblasts in a vaginal birth injury model: influence of the route of administration
Isolation, manipulation and characterization of rat mesoangioblasts (rMABs). (A) rMABs were isolated from 20 days old rat fetuses from skeletal muscle of both hind limbs. After expansion, cells were stained for alkaline phosphatase and further isolated by FACs sorting. In order to track the cells further in vivo, rMABs were labelled with CHMWS-eGFP-T2A-fLuc viral vector and sorted by FACs for GFP expression. The engineered cell line of rMABs GPF+ Luc+ were further validated in vitro by BLI and immunofluorescence. (B) rMABs were differentiated towards skeletal and smooth muscle lineages (left to right). In co-cultures with C2C12 GFP+ rMABs formed few chimeric myotubes (arrow). After smooth muscle differentiation, rMABs expressed both calponin and alpha smooth muscle actin.
