Figure 1 | Scientific Reports

Figure 1

From: Inhibition of Wnt/β-catenin signaling suppresses myofibroblast differentiation of lung resident mesenchymal stem cells and pulmonary fibrosis

Figure 1

Human lung resident mesenchymal stem cells (LR-MSCs) possess MSC characteristics. (A) Morphology of human LR-MSCs was examined by a standard light microscope. (B) Cultured human LR-MSCs were harvested, stained with surface marker antibodies and analyzed with flow cytometry. A representative surface marker profile of human LR-MSCs from one individual is shown. (C) Human LR-MSCs were cultured in osteogenic differentiation medium and differentiated into osteocytes as evidenced by (d) alkaline phosphatase staining at day 7 and (e) alizarin red staining at day 21. Human LR-MSCs were cultured in adipogenic differentiation media for 21 days and stained with (f) oil red O to determine lipid droplet appearance. (a–c) Control cells were cultured in normal growth medium.

Back to article page