Figure 5 | Scientific Reports

Figure 5

From: Hyperthermia induces therapeutic effectiveness and potentiates adjuvant therapy with non-targeted and targeted drugs in an in vitro model of human malignant melanoma

Figure 5The alternative text for this image may have been generated using AI.

Hyperthermia-induced activation of ER stress response pathwaws in human malignant melanoma (A375) cells. The effect of hyperthermia on protein content of PERK, IRE-1a, ATF-6, GRP78/BiP, XBP-1S and CHOP. Cells were grown overnight at 37 °C followed by exposure to hyperthermia, for 2 h, and then transferred back to 37 °C for the indicated post-exposure incubation times (2–72 h). Cell lysates were prepared and subjected to western blotting. Control cells were kept at 37 °C. β-tubulin was used as loading control. Samples from short and long-post exposure incubation periods following hyperthermia were electrophorized on separate gels. Delineation shows blots cropped from different areas of the same blot or different blots. Full-length blots are provided in Supplementary Material (Fig. 3S). Data shown is representative of at least two independent experiments.

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