Table 1 H2O2 accumulation of B. infantis and B. minimum under 0% to 20% O2 conditions.

From: O2-inducible H2O2-forming NADPH oxidase is responsible for the hyper O2 sensitivity of Bifidobacterium longum subsp. infantis

O2 (%)

H2O2 (µM)a

B. infantis wild type

B. infantis ∆npoxA

B. minimum pkkt427b

B. minimum + npoxA in pKKT427c

0

NDd

ND

ND

ND

5

ND

ND

ND

ND

10

29 ± 10

(3 ± 1)e

ND

172 ± 13

20

31 ± 6

14 ± 10

ND

169 ± 18

  1. aH2O2 accumulation in the medium (24 h).
  2. bVector pKKT427 was transformed into B. minimum.
  3. cThe B. infantis npoxA gene was cloned in pKKT427 and transformed into B. minimum.
  4. dND, not detected (less than 3 µM).
  5. eData in parentheses are close to the reliable detection limit.
  6. Data represent the average ± SD of three independent cultures.
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