Figure 1
A/Dose response curve of protein carbonylation induction after chronic irradiation from 0.5 to 6.5 Gy. Worms used in this assay were 3 hours synchronized, irradiated from 0.5 to 6.5 Gy (cumulated doses), and collected. n = 3 (pool of 1000 nematodes). Data are expressed as means of protein carbonylation level (induction relative to unexposed worms) ± SEM (*P < 0.05 Dunnet test). B/Dose response curve of proteasome activity after chronic irradiation from 0.5 to 6.5 Gy. Native gel electrophoresis followed by quantification using IQTL software. n = 3 (pool of 3000 nematodes). Data are expressed as means of proteasome activity (% relative to unexposed worms) ± SEM for the 30S (dark grey), 26S (middle grey) and 20S (light grey) proteasomes as (*p < 0.05; **p < 0.01; ***p < 0.001, Dunnet Test). B/Native gel electrophoresis, followed by in gel proteasome activity assay of the 30S 26S and 20S proteasomes; white line delimits the cropped zone from the same gel (full-length gel is presented in supplementary file Figure S1).
