Figure 2

Microscale tendon properties are altered with dynamic loading. (a) Multiphoton imaging was used to quantify collagen organization (white), amount of F-actin (purple), and nuclear aspect ratio (red) and disorganization (scale bar = 20 µm). (b) Collagen disorganization decreased with applied strain in all groups except those that had been subjected to high/1k loading, similar to (c) the nuclear disorganization. (d) The change in nuclear aspect ratio (ΔnAR) with applied strain also decreased in high magnitude loading groups. *Panels a–c: Data shown as mean ± SD. N = 7–11/group. Lines indicate significant differences. *Panel d: Data shown as mean ± SEM. N = 119–815 cells/group.