Figure 6

Inoculation of TCR^mini mouse with S. aureus drives conversion of CD4⁺ IELs to TCRαβCD8αα lineage. (A) Phylogenetic tree of S. aureus isolated from conventionally reared TCR^mini mice constructed based on comparison of 16S rRNA gene sequence and the concatenated sequences of publicly available S. aureus genome sequences downloaded from the NCBI including 48 completely assembled genomes. (B) Total numbers of CD4⁺ and TCRαβCD8αα⁺ IELs recovered after infection with S. aureus. (C) Selected TCRαβCD4⁺ and TCRαβCD8αα⁺ hybridomas respond to S. aureus lysate by upregulation of Nur77^GFP reporter expression. Overall, we tested 38 hybridomas derived from TCRαβCD8αα⁺ T cells. Abundance of CDR3 sequences from responding hybridomas in populations of TCR^mini mice with indicated microbiological status.