Figure 1 | Scientific Reports

Figure 1

From: Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues

Figure 1

Co-immunoprecipitation of ATP8A2 and ATP8A1 with CDC50A from mouse retina. A retinal extract from mouse retina tissue solubilized in CHAPS detergent was incubated with the immunoaffinity matrix consisting of the Cdc50-7F4 monoclonal antibody covalently coupled to Sepharose 2B. After removal of the unbound fraction, the matrix was washed and eluted with the 7F4 peptide for analysis on SDS gels stained with Coomassie Blue (CB) and western blots labeled with antibodies to CDC50A, ATP8A2 and ATP8A1. The input and unbound lanes contained about 30 µg of protein. Although the P4-ATPases were not detected by Coomassie blue staining due to their low abundance, the ATP8A2, ATP8A1 and CDC50A could be readily detected in the eluted fraction by western blotting.

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