Table 1 Mass Spectrometric Analysis of P4-ATPases in Mouse Tissues.

From: Proteomic Analysis and Functional Characterization of P4-ATPase Phospholipid Flippases from Murine Tissues

Tissue

Protein ID

Protein Name

Mwt (daltons)

Peptides

% Sequence Coverage

% Intensity

Retina

Q8VEK0

CDC50A/TMEM50A

41,061

7

22.5

—

P98200

ATP8A2

129,417

31

32.1

45.3

P70704

ATP8A1

131,413

29

32.3

36.5

Q6DFW5

ATP11B

133,536

21

23.7

12.4

Q9QZW0

ATP11C

129,240

10

12.2

2.9

P98197

ATP11A

135,502

14

15.1

2.9

Liver

Q8VEK0

CDC50A/TMEM50A

41,061

24

61.9

—

Q9QZW0

ATP11C

129,240

114

60.7

95.20

Q148W0

ATP8B1

143,798

29

31.2

1.01

P98197

ATP11A

135,502

26

33.8

0.97

P70704

ATP8A1

131,413

39

41.9

2.54

Q6DFW5

ATP11B

133,536

35

38.0

0.09

Q8K2X1

ATP10D

158,330

10

12.1

0.09

P98200

ATP8A2

129,417

4

4.2

0.06

Brain

Q8VEK0

CDC50A/TMEM50A

41,061

27

49.7

—

P70704

ATP8A1

131,413

77

51.2

56.53

Q9QZW0

ATP11C

129,240

46

38.2

12.85

P98200

ATP8A2

129,417

43

34.7

13.20

Q6DFW5

ATP11B

133,536

42

34.9

8.56

P98197

ATP11A

135,502

36

33.5

8.33

P98199

ATP8B2

136,968

14

18.2

0.27

Q8K2X1

ATP10D

158,330

14

13.9

0.23

Testes

Q8VEK0

CDC50A/TMEM50A

41,061

19

49.7

—

Q6UQ17

ATP8B3

151,955

55

42.2

18.08

Q9QZW0

ATP11C

129,240

53

40.3

23.12

Q6DFW5

ATP11B

133,536

48

43.5

23.97

P70704

ATP8A1

131,413

38

39.6

7.03

P98197

ATP11A

135,502

37

33.8

20.41

P98200

ATP8A2

129,417

37

27.5

5.18

Q8K2X1

ATP10D

158,330

21

18.0

1.25

O54827

ATP10A

168,788

17

15.1

0.93

P98199

ATP8B2

136,968

4

4.6

0.16

Kidney

Q8VEK0

CDC50A/TMEM50A

41,061

23

47.8

—

P98197

ATP11A

135,502

58

37.4

81.12

Q9QZW0

ATP11C

129,240

45

36.7

13.64

P70704

ATP8A1

131,413

32

31.6

2.10

Q6DFW5

ATP11B

133,536

28

25.7

2.99

Q8K2X1

ATP10D

158,330

8

7.5

0.11

  1. A detergent solubilized mouse tissue extract (~20 mg) was applied to a Cdc50-7F4 immunoaffinity column. After removing the unbound protein, the P4-ATPase-CDC50A complexes were eluted with the 7F4 competing peptide. The eluted fraction was subjected to in-gel (retina) or FASP (other tissues) trypsin digestion for analysis of peptides by tandem MS/MS. %Intensity was obtained by taking the fraction of the spectral intensity of a P4-ATPase to the total spectral intensity of all P4-ATPases detected in the tissue.
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