Figure 2
From: Chromatin remodeling in Drosophila preblastodermic embryo extract
Incubation in DREX alters the epigenetic state of chromatin. (a) WB analyses of the global levels of H3Ac after incubation in DREX for the indicated times. Data are presented as normalized mean ± SD (N = 3; two-tailed T-test p-values: *<0.05, **<0.01) (b) H3Ac ChIP-qPCR analysis at the indicated genes after incubation of somatic S2 nuclei in DREX or in control conditions for 1 h. Results are presented as % of input. Data are presented as normalized mean ± SEM (N = 3; two-tailed T-test p-values: *<0.5, **<0–01, ***<0.001). (c) As in (a) but for global levels of H3K4me3. Data are presented as mean ± SD (N = 3; two-tailed T-test p-values > 0.05). (d) H3K4me3 ChIP-qPCR analysis at the promoters of the indicated genes after incubation of somatic S2 nuclei for 1 h in DREX. Results are presented as % of input. Data are presented as normalized mean ± SEM (N = 3; two-tailed T-test p-values: *<0.05, **<0.01, ***<0.001). Full-size films or scans of the blots are presented in Supplementary Figures S8 and S9 (biological replicates).
