Figure 4

Experimental playback setup. The aquarium used for playback experiments was divided into five sections (marked A–E). The playback electrodes were located behind gridded partitions in sections A and E, with the positive pole facing the centre of the aquarium. The focal fish was positioned in the centre, which was divided in space into three equal sections (marked B–D). Sections B and D represent the respective preference zones for A and E; section C was considered a neutral zone. The five (I–V) playback conditions conducted are listed in the table, along with the EOD waveform and SPI combinations. During playback experiments, the actual physical location (section A vs. E) of the synthesized playback combinations was randomly interchanged to control for side-bias. EOD = electric organ discharge; SPI = sequence pulse interval.