Figure 2
From: Enhancing enterocyte fatty acid oxidation in mice affects glycemic control depending on dietary fat
Primary enterocytes from iCPT1mt mice have a higher rate of FAO, mitochondrial respiration and glycolysis than Cpt1mtfl/fl control mice. (A) Rate of oxidation of H3-Palmitic acid by of 250000 (250 K) primary enterocytes isolated from the duodenum or jejunum of Cpt1mtfl/fl and iCPT1mt mice. Left two bars: Mice fed standard chow. Right two bars: Mice fed HFD for 3 days (n = 5–6, Unpaired t test, *P < 0.05, ***P < 0.001, ****P < 0.0001). (B) Oxygen consumption rate (OCR) of primary enterocytes per well, isolated from the (duodenum + jejunum) of Cpt1mtfl/fl and iCPT1mt mice, incubated in KHB medium and subsequently injected with KHB medium or with an effective concentration of 5 mM glucose, 10 µg/mL Oligomycin (Oligo), 8 µmol/L FCCP and 5 µg/mL antimycin + 3.75 µmol/L rotenone (Anti + Rot). (C) Basal respiration, respiration linked to ATP synthesis, maximal respiration, proton leak and spare respiratory capacity of cells in B (n = 5–6, Unpaired t test, **P < 0.01, ***P < 0.001, ****P < 0.0001). (A) Enterocytes from 3 animals were pooled for each genotype (biological replicates) and the n values refer to technical replicates. (B,C) Each well represents a biological replicate of 1, while the n values refer to technical replicates. The data represent values from two independent experiments pooled together. (A–C) Data are presented as mean values ± SEM.
