Figure 3
From: Enhancing enterocyte fatty acid oxidation in mice affects glycemic control depending on dietary fat
Intestinal CPT1mt expression reduces visceral fat with HFD feeding without affecting body weight gain. (A) Body weights of Cpt1mtfl/fl and iCPT1mt mice on CD or HFD monitored over time (n = 11–17; Mixed- repeated measures (RM) ANOVA (time x diet x genotype), ****P < 0.0001 for interaction effects of diet x genotype ($)). (B) CT scan analyses of Cpt1mtfl/fl and iCPT1mt mice fed CD or HFD for 19 weeks (n = 5–10; 2 × 2 factorial ANOVA (diet x genotype). *P < 0.05, ****P < 0.0001 for main effect of diet and post hoc tests (dashed lines) and interaction effects of diet x genotype ($). (C) Representative bright field microscopy images of H&E stained duodenum, jejunum and liver sections of Cpt1mtfl/fl and iCPT1mt mice fed CD or HFD for 20 weeks at 20X magnification. The yellow bar represents a 100 μm scale. (D) Measurements of the villi lengths of Cpt1mtfl/fl and iCPT1mt mice from H&E stained sections. Left: Duodenum, Right: Jejunum (n = 3–5; 2 × 2 factorial ANOVA (diet x genotype). *P < 0.05, **P < 0.01 for main effect of diet and post hoc tests (dashed lines) and interaction effects of diet x genotype ($)). (A,B,D) Data are presented as mean values ± SEM.
