Figure 4
From: A Novel Multi-Gene Detection Platform for the Analysis of miRNA Expression
Dynamic range of known concentration of spike-in synthetic has-miR-10a-5p. A set of 10-fold serial dilutions of synthetic hsa-miR-10a-5p miRNA were spiked into the same amount of Universal Human miRNA reference RNA (samples 16–22, miRQC A) to generate cDNA for qPCR analysis. 20 ng of the RNA input was used for the spike-in test. qPCR results illustrated a dynamic range of at least 7 orders of magnitude ranging from 80 to 8 × 107 copies per nanowell.
