Figure 2 | Scientific Reports

Figure 2

From: Potent Attractant for Root-Knot Nematodes in Exudates from Seedling Root Tips of Two Host Species

Figure 2

Response of root-knot nematodes to root exudates collected from Pluronic (PF-127) gel. (a) Tomato seedlings were aligned in a square, 100 mm Petri plate containing 25 ml of 23% PF-127 gel. Zones are indicated from which gel was collected after 24 hr exposure to seedlings. (b) A two well assay was performed in a Petri dish (60 mm). Infective juveniles (J2) were suspended in the gel. Wells were filled with PF-127 gel that had been exposed to root exudate or to controls. The number of juveniles in the wells was counted after 6 hr. Panel (c) shows numbers of J2 of Meloidogyne javanica (Mj), M. incognita (Mi) or M. hapla (Mh) accumulating in assay wells to which gel had been transferred from Zone 1 or Zone 3 (n = 6). Panels (d) and (e) compare accumulation of M. hapla and M. javanica J2, respectively, in wells receiving gel from Zone 1, Zone 2, and Zone 3 (n = 4). For each graph, letters above bars indicate significantly different numbers of J2 (P < 0.05) according to Tukey’s multiple range test.

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