Figure 3 | Scientific Reports

Figure 3

From: Identification of ABCG2 as an Exporter of Uremic Toxin Indoxyl Sulfate in Mice and as a Crucial Factor Influencing CKD Progression

Figure 3

ABCG2-mediated transport of uremic toxins. (a) In vitro ATP-dependent transport activities of indoxyl sulfate (IS), hippuric acid (HA), and creatinine (CTN) by membrane vesicles prepared from HEK293A cells expressing Myc-human ABCG2 or EGFP. The substrate concentration of IS and HA was 30 µM and that of CTN was 300 µM. Open and closed columns indicate values in the absence and presence of ATP, respectively (mean ± S.E., n = 3–4 for each experimental condition). **P < 0.01 (non-repeated ANOVA followed by Student-Newman-Keuls test). (b) Concentration-dependent transport of IS by ABCG2 vesicles. Symbols and bars indicate mean and S.E., respectively (n = 3–4), and the dotted line indicates fitting line. Each value was calculated by subtracting the transport activity in the absence of ATP from that in the presence of ATP.

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