Figure 9

Shotgun metagenomics sequencing was used to confirm the enrichment of specific ABR genes in the classes of aminoglycosides, beta-lactams and tetracycline to the relative enrichment of specific species of the Enterobacteriaceae observed in the 16S amplicon sequencing results. ABR genes were linked to the enrichment of specific Enterobacteriaceae genes obtained with shotgun metagenomics sequencing, thus confirming the results found in the 16S sequencing, ABR microarray and in silico CARD analysis. The heat map scale represents a log2 (+1) transformed value of the abundance of ABR genes from shotgun metagenomics sequencing.