Figure 5
Comparisons of damage and crypt cell motion after single cell ablation in young mice with and without Y-27632, and aged mice. (a–f) Comparison of crypt cell behavior in untreated, inhibited, and old mice. Red color indicates untreated, young mice, blue color is young, Y-27632 treated mice, and green color indicates old mice with no treatment. At each measurement time, dots are spaced for visual clarity. (a,b) Area labeled with propidium iodide (PI) after ablation at the crypt base (a) and at the upper layer (b). Mean and SD are plotted at each time point and dots represent individual crypts. *Indicates comparison between untreated, young mice and old mice, #between Y-27632 treated, young mice and old mice, and + between untreated young mice and Y-27632 treated, young mice. **,##,++Indicates p < 0.01, and *, #,+indicates p < 0.05 (2-way ANOVA with Tukey’s multiple comparisons). (c) Sum of distance moved by Paneth cells at the crypt base between baseline and one hour after ablation. Mean and SD are plotted with dots representing individual Paneth cells. Significance calculated with ANOVA with Tukey’s multiple comparisons. (d–e) Measurement of inner lumen (d) and outer circumference (e) at the crypt at each time point after ablation. Each value was normalized to the baseline. Mean and SD are plotted with dots representing individual crypts. Black indicates measurements in control crypts adjacent to ablated crypts. *Indicates comparison between untreated, young mice and Y-27632 treated, young mice, and #indicates comparison between untreated, young, ablated mice and untreated, young, unablated mice. ***,###Indicates p = 0.0003 (2-way ANOVA with Tukey’s multiple comparisons). (f) Position of cellular debris along the crypt lumen. Dots represent individual crypts at each time point. Lines represent linear regressions (R2 = 0.9665 for untreated, 0.953 for inhibitor, and 0.1687 for old).
