Table 2 CCD with observed and predicted values of CO activity.

From: Response Surface Methodology-Genetic Algorithm Based Medium Optimization, Purification, and Characterization of Cholesterol Oxidase from Streptomyces rimosus

Yeast Extract

Dextrose

Starch

Ammonium carbonate

Enzyme activity U/ml

Observed

Predicted

Residual

0.4

0.4

0.8

0.02

1.694

1.698545

−0.004545

0.4

0.4

0.8

0.02

1.7

1.698545

0.001455

0.6

0.6

0.4

0.01

2.168

2.164430

0.003570

0

0.4

0.8

0.02

0.66

0.788276

−0.128276

0.2

0.2

0.4

0.01

0.76

0.749693

0.010307

0.4

0.4

1.6

0.02

1.96

2.097274

−0.137274

0.4

0.4

0.8

0

1.25

1.326244

−0.076244

0.8

0.4

0.8

0.02

2.788

2.823809

−0.035809

0.2

0.2

1.2

0.01

1.534

1.394519

0.139481

0.4

0.4

0.8

0.02

1.7

1.698545

0.001455

0.4

0

0.8

0.02

1.33

1.402118

−0.072118

0.4

0.4

0.8

0.04

1.8

1.854525

−0.054525

0.6

0.2

1.2

0.03

2.427

2.344019

0.082981

0.6

0.2

0.4

0.03

2.023

2.019173

0.003827

0.4

0.8

0.8

0.02

1.926

1.977144

−0.051144

0.4

0.4

0.8

0.02

1.69

1.698545

−0.008545

0.4

0.4

0.8

0.02

1.7

1.698545

0.001455

0.4

0.4

0

0.02

1.35

1.283244

0.066756

0.4

0.4

0.8

0.02

1.71

1.698545

0.011455

0.4

0.4

0.8

0.02

1.7

1.698545

0.001455

0.4

0.4

0.8

0.02

1.69

1.698545

−0.008545

0.2

0.6

1.2

0.03

1.855

1.743433

0.111567

0.2

0.6

0.4

0.03

1.2

1.193526

0.006474

0.6

0.6

1.2

0.01

1.939

1.832878

0.106122

0.4

0.4

0.8

0.02

1.71

1.698545

0.011455

0.4

0.4

0.8

0.02

1.7

1.698545

0.001455

0.4

0.4

0.8

0.02

1.69

1.698545

−0.008545

  1. Note: The concentration units of yeast extract, dextrose, starch and ammonium carbonate were in g/100 ml.
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