Table 1 Restriction-Modification systems and dam/dcm methylases present in the genomes of Psa3 (ICMP18708) and Psa1 (ICMP9853).

From: Comparison between complete genomes of an isolate of Pseudomonas syringae pv. actinidiae from Japan and a New Zealand isolate of the pandemic lineage

REBase name

type

Psa3, ICMP18708

position in genome

Psa3, Hym1

Psa3, Jilo4

M.Psy708NZORF25P

Type I

AGCANNNNNGTC

6,593–12,826

present

absent

M.Psy708NZORF130P

Type I

disrupted by ISPsy34

33,116–41,522

present but disrupted

present, no disruption

M.Psy708NZORF855P

Type II

CTCGAG

181,850–202,419

  

RM.Psy708NZORF5205P

Type II

non-functional homologue of RM-Psy9853ORF25530P, disrupted by two MITEs and ISPsy28

1,105,031–1,107,495

 

disrupted by one MITE and ISPsy28

M.Psy708NZORF8545P

Type III

disrupted by in-frame stop and a MITE

1,837,995–1,845,322

 

disrupted by in-frame stop

M.Psy708NZORF24760P

dcm

CC(A/T)GG

5,495,131–5,496,546

  
  

Sequence similarity suggests this a dcm methylase; it is present within the Pac_ICE1 element

   

REBase name

type

Psa1, ICMP9853

position in genome

  

M.Psy9853I/Psy9853IP

Type II

CTGCAG

1,702,030–1,704,452

  

RM.Psy9853ORF25530P

Type II

Deletion of 951 bp, homologue of RM-Psy708NZORF5205P

5,320,330 - 5,318,258

  

M.Psy9853II/Psy9853IIP

Type III

TCCACC

6,024,961–6,029,546

  

M.Psy9853ORF28855P

dam

GATC

6,032,376–6,033,170

  
  

Sequence similarity suggests this is a dam methylase; it is present in an integrated prophage

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