Figure 6
From: A novel triple combination of pharmacological chaperones improves F508del-CFTR correction
Differential scanning fluorimetry indicates that MCG1516A can interact directly with NBD1. (A) Representative melting curves for human NBD1 determined in the presence of increasing ATP concentrations. (B) Representative F508del-NBD1 melting curve for human NBD-1 in the presence of VX-809 (1 µM) (C) Unfolding of F508del-NBD1 is unaffected by the presence of latonduine. (D) Representative F508del-NBD1 melting curve in the presence of MCG1516A. Also shown is the signal obtained with MCG1516A and Sypro orange dye alone (no protein). (E) Effect of buffer, 1% DMSO or 10 μM MCG1516A on F508del-CFTR stability in the presence of increasing amounts of ATP. In all parts of the figure the error bars indicate SEM (with an n = 3).
