Figure 7
From: A novel triple combination of pharmacological chaperones improves F508del-CFTR correction
Effects of RDR1, VX-809 and MCG1516A on F508del-CFTR when tested individually and in combination. (A) Surface expression of F508del-CFTR with 10 μM MCG1516A (1516a), 10 μM RDR1 and 1 μM VX-809 when pretreated for 24 h separately and combined using the CSEA assay. (B) Immunoblot of F508del-CFTR in BHK cells after the same treatments as in A. (C) A graph of the relative band intensity of band C when compared to the combined intensities of bands B and C in panel B. (D) FLIPR membrane potential assay (FMP) results in which correction by treatments shown in A is measured functionally as the depolarization that results from F508del-CFTR channel activation by genistein + forskolin.
