Figure 3 | Scientific Reports

Figure 3

From: Novel interactomics approach identifies ABCA1 as direct target of evodiamine, which increases macrophage cholesterol efflux

Figure 3

Interactions between polyclonal anti-ABCA1 antibody, evodiamine and ABCA1 protein (ABCA1 peptide from Abcam, #ab125995, covering C-terminal domain 2085–2245aa). (a) Interaction of polyclonal anti-ABCA1 antibody with immobilized ABCA1 on CM5 sensor chip as assessed by Biacore 3000. The interaction affinity is calculated with association constant (KA) equal to 1.18e6M and dissociation constant (KD) equal to 4.57e-7M (antibody concentrations from bottom to top are 4.25e-8, 8.5e-8,1.75e-7, 3.5e-7 and 7e-7M). (b) Interaction affinity between evodiamine and ABCA1. Evodiamine interacts with an association constant (KA) equal to 1,23e6M and a dissociation constant (KD) equal to 8,1e-7M (evodiamine concentrations from bottom to top are 2.5e-7, 5e-7 and 1e-6M). There is a bulk effect with Evodiamine at 1e-6M. This explains the higher signal (RU) in regard to the two lower concentrations. (c) Interaction of retinal dehydogenase-1 (ALDHA1) with immobilized ABCA1 peptide. The two proteins interact with a KA equal to 1.25e6M and KD of 8.03e-7M (ALDHA1 concentrations from bottom to top are; 6e-8, 1.25e-7, 2.5e-7, 5e-7M and 1e-6M).

Back to article page