Figure 2

Examples of relatively simple networks and the corresponding chloroplasts hosting them are displayed. Confocal image z-stacks were deconvolved via Huygens software (Scientific Volume Imaging). For illustration, maximum intensity projection (MIP) and volume reconstruction (blend rendering) were applied to the deconvolved images via the IMARIS software (Bitplane). (a) FtsZ1-2 networks are characterised by extensive extraplastidic filaments. (b) FtsZ2-1 networks are interspersed with occasional meganodes (arrowheads). Visual analysis of the 3D images of the chloroplasts and FtsZ networks also reveals that the chloroplasts of the transfected cells often show morphological divergence from the standard chloroplast shape. This implies that elevated levels of FtsZ might induce morphological changes on the chloroplasts. Interestingly, the morphological deformation tends to have certain patterns which are isoform-specific.