Figure 3
Analysis of migrational properties of BMDCs from WT and NO-GC1-deficient mice. (A) Proportion of migrated cells towards CCL19 in the transwell assay. Spontaneous migration (without CCL19) referred to as control (ctrl). (B) Relative amount of CCR7/CD197 expressing BMDCs in FACS analysis. (C) Relative quantification (RQ) of CCL19 mRNA expression in BMDCs as measured with Real Time PCR. (D) Proportion of migrated BMDCs towards CCL19 with and without 24 h preincubation with a neutralizing anti-CCL19 antibody. Spontaneous migration (without CCL19) referred to as control (ctrl). Data are mean ± SEM of (A) n = 9/group from four independent experiments and (B–D) n = 4–6/group from two independent experiments. Two-way ANOVA with subsequent Bonferroni’s post test or Student’s test were performed. *P < 0.05, **P < 0.01, ***P < 0.001.
