Figure 4

BMDCs from NO-GC1 mice display increased cAMP formation and Th1 bias. (A) NO-GC activity as measured in cGMP formation and (B) cAMP levels in lysates of WT and NO-GC1 KO BMDCs without stimulation (basal) and after treatment with the AC activator Forskolin (Fsk). (C) Th1 cytokine level (ELISA) in supernatant of cocultured naïve OT2 T-cells with either WT or NO-GC1 KO BMDCs with or without cGMP pretreatment. Data are mean ± SEM of n = 2 (B) or n ≥ 3 (A + C) independent experiments. Mann-Whitney test (A), One-way ANOVA with subsequent Dunn’s post test (B) or two-way ANOVA with subsequent Bonferroni’s post test (C) were performed. *P < 0.05, **P < 0.01, ***P < 0.001.