Figure 9

Quantitative RT-PCR validation of PIRL. (a) The control experiment of clean water. CK1, T1, T2, T3, and T4 indicated that after the treatment with sterile water/B. cinerea at 0 h, 48 h, 72 h, 96 h and 120 h, respectively. (b) The treatment experiment of B. cinerea. The qRT-PCR reaction was repeated three times, and the template amount was corrected by strawberry 26S rRNAs. The normalized miRNA levels at CK1 were arbitrarily set to 1. The vertical bar indicates standard error.