Figure 1

Dynamics of actin re-polymerization on SHEDs. At left, representative images of cells with depolymerized actin filaments right after incubation with ROCKi (time 0 h) at 20x magnification (A), for the control sample F5541 (B) and for the ASD sample F7511 (C). At right, representative images of cells with re-polymerized filaments after 60 minutes of Cdc42 activation treatment at 20x magnification (D), for the control sample F5541 (E), and for the ASD sample F7511 (F). It is possible to notice much more cells with a large cell body due to stress fibers formation in the control sample (E), whereas in patient, there are much more cells with a thin cell body with no stress fiber formation (F). Scale bar: A and D: 50um; B,C, E and F: 200um.