Figure 1

(A) Expression profiles of genes relevant to breast microcalcifications that are differentially expressed in metastatic and non-metastatic breast cancer cell lines. The gene expression heat map focused on genes directly linked to microcalcifications was obtained by subjecting the publicly available microarray dataset GSE16795 to the Gene-e matrix visualization and analysis platform. From the 38 cell lines included in the dataset, 5 non-metastatic and 5 metastatic cell lines with high relevance to our experimental work were identified and utilized to generate the heat map. (B) Protein-protein interaction network of differentially expressed genes in the selected metastatic versus non-metastatic cell lines. Identified biological processes and pathways that involve OPN (SPP1) are shown. Network nodes are colored by pathway membership, and pathways are sorted by increasing false discovery rate. Interactions are colored by type of interaction as listed in the legend. Solid and dotted lines represent intra-cluster and inter-cluster functional associations, respectively.