Table 1 Sequences of primers used in this study.
From: Cyclooxygenase-2 up-regulates hepatic somatostatin receptor 2 expression
Target | Sequence | Size (bp) |
|---|---|---|
Rat SSTR-2 | F: 5′-GAAAAGCAAGATGTCACGATAG-3′ R: 5′-TTGGCTCCCATTGAACTG-3′ | 135 |
Rat COX-2 | F: 5′-GCTCATACTGATAGGAGAGACGA-3′ R: 5′-TGGAACTGCTGGTTGAAAAG-3′ | 117 |
Rat GAPDH | F: 5′-TCGGTGTGAACGGATTTG-3′ R: 5′-CTCAGCCTTGACTGTGCC-3′ | 173 |
Bisulfite sequencing | F: 5′-AAACTACCCTAACCTATAAATCAT-3′ R: 5′-GGTTTTGTAATTTGTGTTTTGTT-3′ | 499 |
Sequenom MassARRAY (1) | F: 5′-GGGGTTTTTATTTATTTATTGGGGT-3′ R: 5′-TAAACTCAAACCTCCTCCTACCTCT-3′ | 487 |
Sequenom MassARRAY (2) | F: 5′-TTGAGGAGATTTGAATTTAGAATGG-3′ R: 5′-AAAAAACCAAAAAATAAAAACAACC-3′ | 356 |
Sequenom MassARRAY (3) | F: 5′-TTGTTAGTTATTGTTTGTTGGTTTGA-3′ R: 5′-CCACCCAAATACACTCTCTATCTCT-3′ | 347 |
Sequenom MassARRAY (4) | F: 5′-GTTGGGGTTGGGTTAGATTAGTAAG-3′ R: 5′-TTTTACCCAACAAATTACAAACAACT-3′ | 358 |
Sequenom MassARRAY (5) | F: 5′-GGTGTTTAAAGTGTGTTGTTTTTTTT-3′ R: 5′-CTCCTCACCAAATCCTACAAAACT-3′ | 470 |
Sequenom MassARRAY (6) | F: 5′-GTAGGGAGGGATAATATATTTTGGG-3′ R: 5′-ACACTTAACATACCCCTTCTCCTTT-3′ | 339 |
