Figure 3 | Scientific Reports

Figure 3

From: Coactivation of MEP-biosynthetic genes and accumulation of abietane diterpenes in Salvia sclarea by heterologous expression of WRKY and MYC2 transcription factors

Figure 3

Identification and characterization of transcription factors likely involved in the regulation of genes encoding enzymes of the MEP-pathway in A. thaliana. Localization of representative W-box and G-box binding sites in the WRKYs and MYC2 promoters of A. thaliana MEP-pathway biosynthetic genes (a). Methyl-Jasmonate Responsive Elements (MJRE), W-box and G-box were present also in the 1000 bp region upstream the transcription start site (TSS) of the AtMYC2, AtWRKY18 and AtWRKY40 (b). Time–course transcriptional activation of AtWRKY18 (c), AtWRKY40 (d) and AtMYC2 (e) TFs in A. thaliana seedlings in response to elicitation with MJ 150 µM for 24 h, measured by qRT-PCR and expressed as fold-changes compared to the expression level of untreated control seedlings. Values are means ± SD of three technical repeats from three biological replicates.

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