Figure 3
HTLV-1 expressing cells form TNTs with non-infected T cells and monocytes and contain Tax. (A) THP-1-memGFP and Jurkat-memCherry cells were co-cultured overnight and presence of TNTs was analyzed by DIC and fluorescent confocal microscopy. Arrows indicate TNTs and arrowheads memCherry and memGFP. (B) MT-2 (unstained) and Jurkat-memCherry cells were co-cultured overnight and the presence of TNTs was analyzed by DIC and fluorescent confocal microscopy. Arrows indicate TNTs and arrowheads memCherry. (C–E) MT-2 and THP-1-memGFP cells were co-cultured overnight and immunostained for Tax (red). The presence of TNTs was analyzed by DIC and fluorescent confocal microscopy. Arrows indicate TNTs and arrowheads memGFP (green) or Tax (red). All images are representatives of two independent experiments performed in duplicates. The X-Z plane is obtained from a Z-stack (total 32 slices of 1 µm each). Scale bar 10 µm. Adobe photoshop CS6 was used to prepare the images. The contrast was enhanced on the whole image to better represent and visualize the thin TNT structures.
