Figure 4

Wdfy3 β-gal reporter expression in Wdfy3^+/lacZ mice Top panel: Progressively more posteriorly located coronal brain sections of 3-m old (PND100) Wdfy3^+/lacZ mice reveal β-gal expression under Wdfy3 control in brain areas specifically examined for mitochondrial dysfunction. Anteriorly (A), strongest expression can be seen in accessory olfactory bulb (AOB) and dispersed cells within external plexiform (EPL) and glomerular layers (GlL) of olfactory bulb (OB). In cortex (B,C) dispersed β-gal⁺ cells can be seen throughout, but condensed in the piriform cortex (PCx). Pronounced β-gal expression can also be detected in the hippocampal (HC) CA3 region (C), choroid plexus (CP), and cerebellar Purkinje cell layer (PCL, D). Bottom panel: WT brain processed for X-gal staining reveal absence of β-gal expression. Antero-posterior locations line up in (A’) with olfactory bulb (OB), (B’) striatum (Str), (C’) anterior hippocampus (HC), and (D’) cerebellum. Abbreviations: IV. fourth ventricle; AC, anterior commissure; AOB, accessory olfactory bulb; CC, corpus callosum; DG, dentate gyrus; EC, external capsule; Fi, fimbria; GCL, granular cell layer; GIL, glomerular layer; LV, lateral ventricle; MB, midbrain; Me, medulla; ML, molecular layer; Str, striatum; Th, thalamus; WM, white matter. Scale bar is 500 µm.