Figure 3
Detection of DCS after CQ treatment. DCS appeared in the cytosol of CQS parasites after CQ exposure. These structures were scarce in CQR parasites, even after high [CQ] exposure. (A), CQS (3D7, HB3) and CQR (Dd2, FCB) strains were incubated with 500 nM CQ, 500 nM CQ + 10 µM VP, or Ringer’s solution only (ctrl) for 2–3 h. DCS (arrows) were only found in the cytosol of CQS parasites treated with CQ. Scale bar, 2 µm. (B) 3D7 and Dd2 parasites were incubated with various [CQ] and the percentage of parasites with DCS was determined at 30 min intervals. Results are from three independent experiments; more than 3,000 parasites were evaluated. Error bars represent SEM. (C) correlation between parasite survival and number of 3D7 (black circles) and Dd2 parasites (open triangles) containing DCS at the respective survival rates. Results are from three independent experiments. Error bars represent SEM.
