Figure 4
Electron microscopy images of CQ-exposed 3D7 parasites. 3D7 parasites were exposed to 20x IC50 CQ for 3 h, fixed and prepared for EM. (A), Hz crystals were detected in cytosolic compartments. Magnifications of the images show the intracellular localization of the Hz-containing structures and the surrounding membrane bilayer. (B) multiple undigested membranes are detected in the DV. (C) large vacuoles form in the parasite cytosol and exceed the size of the DV. Images were obtained at 15,000x magnification and digital zoom. Scale bars: i, 1 µm; ii, 200 nm; iii, 50 nm. Arrowheads: membrane bilayers. DV, digestive vacuole.
