Table 1 Summary of phenotypic analysis.
Screen | Method | Phenotype summary Hes 3 null |
|---|---|---|
NORMAL DIET | ||
Energy Metabolism | Indirect calorimetry NMR | -None -Trend towards increased fat content over time mainly in male mutants |
Behavior | Open field | -None |
Acoustic startle response, PPI | -None | |
Neurology | Modified SHIRPA, Auditory brain stem response Rotarod Grip strength | -None -None -None -Small trend towards increased fore paw force |
Nociception | Hot plate | - Shorter reaction time for the first pain reaction in females, trend towards hyperalgesia |
Dysmorphology | Anatomical observation, X-ray, MicroCT scans (dissected bones) | -None |
Cardiovascular | Awake ECG Echocardiography | -No clear phenotype -Very mild increase in septum width in systole (males). -Very mild reduction in heart rate and thus RR interval prolongation -Very mild alterations in QRS, ST and Qtdisp intervals (probably by chance) |
Eye | Scheimpflug imaging, OCT, LIB, drum | -Slight decrease in retinal thickness (females) |
Clinical Chemistry | IpGTT Insulin levels Clinical chemical analysis Hematology | -IPGTT: Mild trend downwards in males and upwards in females for AUC values. -None -Trend towards changes in creatinine and fructosamine (mainly females) concentrations -Hematology: Slightly lower platelet distribution width |
Immunology | Flow cytometry analysis of Peripheral Blood Leukocytes | -Subtle alterations in the leukocyte subpopulations, however no evidence for pathological effects in the immune system: -increased frequency of B cells -decreased frequency of CD4 single positive T cells -increased frequency of CD4 CD8 double positive T cells -increased proportion of CD8 single positive T cells (females) -increased CD44 expression on CD4+ T cells |
Allergy | ELISA (IgE concentration) TEWL | -None -None |
Pathology | Macro & microscopic analysis | -None |
HIGH FAT DIET | ||
Energy Metabolism | Indirect calorimetry, NMR | -None |
Body temperature | -Slight increase in males and females | |
Fat mass | -Mild decrease in females | |
Allergy | TEWL | -Slight increase in females |
Body surface temperature | -None | |
Clinical Chemistry | IpGTT | -IPGTT: Mild trend downwards in males for AUC values. |
