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Figure 1

From: Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem

Figure 1

Purification and glycosylation state of AcmJRL. (a) Affinity chromatography of AcmJRL on D-mannose-Agarose, the arrow indicates the starting elution with D-mannose. (b) FPLC-gel filtration chromatography on Superdex 75 of the affinity-purified AcmJRL. Inset 1: SDS-PAGE. Lane 1: molecular weight standards, lane 2: total protein soluble fraction of A. comosus stem, lane 3: affinity chromatography flow-through fraction, lane 4: affinity chromatography retained fraction, lane 5: FPLC-gel filtration chromatography of the affinity chromatography retained fraction. Inset 2: native PAGE of AcmJRL after FPLC-gel filtration chromatography. (Glycan detection experiments on AcmJRL. (c) SDS–PAGE and (d) glycoprotein detection. 1 and 8: molecular weight standards, 2: positive control (horseradish peroxidase), 3–6: different preparations of AcmJRL, 7: negative control (soybean trypsin inhibitor).

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