Figure 3 | Scientific Reports

Figure 3

From: Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem

Figure 3

Isothermal titration calorimetry of D-mannose binding to AcmJRL and Enthalpy-entropy compensation plot. (a) Upper panel: exothermic microcalorimetric traces of D-mannose (32 mM) injections into AcmJRL solution (0.8 mM). Lower panel: Wiseman plot of heat releases versus molar ratio of injectant/protein in the cell and nonlinear fit of the binding isotherm for 2 equivalent binding sites. (b) Enthalpy-entropy compensation plot of sugar binding to AcmJRL. Enthalpy and entropy values are from Table 2. The plot displays a linear relationship with r2 = 0.97.

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