Figure 5
Multiple sequence alignment of relevant mJRLs. The alignment of AcmJRL, Banlec, artocarpin, heltuba and calsepa was obtained using Clustal W and improved thanks to the superimposition of the corresponding structures. Arrows above the alignment represent the β-strands of AcmJRL. The residues involved in D-mannose binding in site 1 and 2 are highlighted in yellow and orange, respectively. Residues binding the 3rd D-mannose in Banlec are highlighted in green. Residues involved in side-by-side dimerization and higher order oligomerization (octamer for heltuba and tetramer for others) are highlighted in grey and blue respectively. The four residues forming the “wall” between the binding sites of AcmJRL and Banlec are showed in red.
