Figure 6

Interactions of the α-D-mannose and methyl-α-D-mannopyranoside in the carbohydrate-binding sites of AcmJRL and comparison with BanLec. (a) Monomer A of the AcmJRL:Man complex is shown in cartoon representation with rainbow coloring from the blue N-terminus to the red C-terminus. The D-mannose molecules are shown as yellow sticks, and the amino acids involved in their binding as grey sticks. The D-mannose binding site 1 and 2 are formed by the loops of the Greek key 1 and 2 respectively. Two inserts highlight the electron density of the feature-enhanced map at the 1σ level around the two D-mannose molecules. (b) Same as A for the AcmJRL:MeαMan complex, the methyl-mannose molecules being displayed in green. (c) Superposition of AcmJRL and BanLec in complex with methyl-α-D-mannopyranoside. AcmJRL is represented in grey cartoon and sticks and BanLec in orange. MeαMan molecules in complex with AcmJRL and BanLec are shown as green and yellow sticks respectively. The close contact between MeαMan1 and Glu14 in AcmJRL is highlighted in magenta. (d) Comparison of the β7β8 loops of AcmJRL and BanLec with the same representation style as (c). Thr84 of the BanLec His84Thr mutant is shown in yellow sticks.