Figure 3
From: Gonadal steroid levels in rock pigeon eggs do not represent adequately maternal allocation
Digestion of yolk proteins with proteinase-K at a final concentration of 2.0 mg/ml for 3 hours at 37 °C as visualized on 12% Tris-Glycine gel. Lane 1 shows bands of a commercial protein molecular weight marker (ThermoFisher Scientific). Yolk proteins are shown before (lanes 2, 4, 6) and after (lanes 3, 5, 7) proteinase-K treatment for three fractions – crude (lanes 2 and 3), supernatant (lanes 4 and 5), and pellet (lanes 6 and 7). Lanes 1 and 2–7 are cropped from the same gel which is provided in Fig. S1 in the supplementary information.
